DOT stands for Department of Transportation and HHS stands for Health and Human Services. The DOT and HHS have very strict rules on how to conduct pre-employment, return-to-duty, post-accident, follow-up, reasonable suspicion, random etc. drug tests.

Here is a quick breakdown of the procedural requirements in DOT testing:

Pre-DOT Collection Procedures:

Inspect collection site, secure water sources, add bluing agent to both toilet and tank, remove soap or foreign substances, forbid unauthorized access and remove all trash receptacles.

Step #1: Donor must provide valid photo ID
Why: To ensure that donor has not sent a substitute in his/her place

Step #2: Explain collection procedures
Why: To prevent misunderstanding and/or confusion

Step #3: Instruct donor to remove outer clothing and empty pockets
Why: To assess whether potential adulteration materials were brought to the collection site by donor

Step #4: Instruct donor to wash hands under collector supervision, with liquid soap only, followed by immediate removal of soap dispenser from bathroom
Why: To hamper their ability to conceal adulterants in the palm of their hand or even under the fingernails

Step #5: Instruct donor to void bladder and hand specimen to collector immediately after void, before flushing the toilet or washing hands again
Why: Collector must check temperature within 4 minutes

Step #6: Evaluate specimen for potential adulteration (i.e., temperature out of range, dilution, odor of bleach and/or peroxide, etc.)
Why: If any adulteration attempts are determined, a 2nd directly-observed test must be conducted immediately

Step #7: Check volume and prepare specimens for shipping to lab in the presence of donor
Why: Urine specimen must be visible by both collector and donor throughout the specimen preparation process to avoid future confusion or statements made by donor that the collector has altered/substituted sample

Step #8: Complete paperwork and provide donor a copy while instructing him/her to list any over-the-counter or prescription medications they might be taking
Why: If a drug category reports as positive, an MRO (Medical Review Officer) will contact donor to confirm the positive result and determine any potential “false positives”

The following are the testing authorities under which DOT regulations must be adhered to:

FMCSA (Federal Motor Carrier Safety Administration)
FAA (Federal Aviation Administration)
FRA (Federal Railroad Administration)
FTA (Federal Transit Administration)
PHMSA (Pipeline and Hazardous Materials Safety Administration)
USCG (United States Coast Guard)

HHS (US Dept. of Health and Human Services)
DOE (Department of Education)
NASA (National Aeronautics and Space Administration)
DOL (Department of Labor)
FBI (Federal Bureau of investigation)

NRC (Nuclear Regulatory Commission)

Editor’s Note: ADS performs all DOT and HHS federal testing as listed above and also adheres to DOT 49 CFR Part 40 Regulations for all rapid and NON-DOT urine testing. We also perform nationwide criminal background checks and motor vehicle reports, as they are often required along with DOT drug testing.

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